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  • A mutation in the gene for polynucleotide kinase of

    Aug 15, 2013 · Dudas KC, Kreuzer KNJ (2005) Bacteriophage T4 helicase loader protein gp59 functions as gatekeeper in origin-dependent replication in vivo. J Biol Chem 280:21561–21569. doi: 10.1074/jbc.M502351200 CAS PubMed Central PubMed Article Google Scholar

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  • Bacteriophage T4 Helicase Loader Protein gp59 …

    Structural analysis of bacteriophage T4 DNA replication: a

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  • Bacteriophage T4 helicase loader protein gp59 functions as

    Structural analysis of bacteriophage T4 DNA replication: a

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  • Enterobacteria phage T4 - Labome.Org

    Dudas K, Kreuzer K. Bacteriophage T4 helicase loader protein gp59 functions as gatekeeper in origin-dependent replication in vivo. J Biol Chem. 2005;280:21561-9 pubmed publisher b>Bacteriophage T4 initiates origin-dependent replication via an R-loop mechanism in vivo

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  • Conditional coupling of leading-strand and lagging-strand

    Jun 04, 2001 · Bacteriophage T4 helicase loader protein gp59 functions as gatekeeper in origin-dependent replication in vivo. Dudas KC, Kreuzer KN. J Biol Chem, 280(22):21561-21569, 21 Mar 2005 Cited by: 19 articles | PMID: 15781450 | PMCID: PMC1361368. Free to read

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  • Two-dimensional gel analysis of rolling circle replication

    Jun 01, 1996 · Bacteriophage T4 helicase loader protein gp59 functions as gatekeeper in origin-dependent replication in vivo. Dudas KC, Kreuzer KN J Biol Chem, 280(22):21561-21569, 21 Mar 2005

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  • Coordinated DNA Replication by the Bacteriophage T4 Replisome

    A model of the T4 bacteriophage DNA replisome. Replication of T4 genomic DNA is accomplished by a replication complex composed of eight proteins. The helicase (gp41) and primase (gp61) interact to form the primosome with the assistance of the helicase loader (gp59).

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  • Enterobacteria phage T4 - Labome.Org

    Dudas K, Kreuzer K. Bacteriophage T4 helicase loader protein gp59 functions as gatekeeper in origin-dependent replication in vivo. J Biol Chem. 2005;280:21561-9 pubmed publisher b>Bacteriophage T4 initiates origin-dependent replication via an R-loop mechanism in vivo

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  • Investigation of stoichiometry of T4 bacteriophage

    Investigation of stoichiometry of T4 bacteriophage helicase loader protein (gp59). Arumugam S.R., Lee T.H., Benkovic S.J. The T4 bacteriophage helicase loader (gp59) is one of the main eight proteins that play an active role in the replisome. gp59 is a small protein (26 kDa) that exists as a monomer in solution and in the crystal.

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  • Control of homologous recombination by the HROB-MCM8 …

    The function of HROB in HR is reminiscent of that of gp59, which acts as the replicative helicase loader during bacteriophage T4 recombination-dependent DNA replication. We therefore propose that the loading of MCM8-MCM9 by HROB may similarly be a key step in the establishment of mammalian recombination-associated DNA synthesis.

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  • Structural analysis of bacteriophage T4 DNA replication: a

    Dec 03, 2010 · The T4 primosome is composed of the gp41 hexameric helicase, the gp59 helicase loading protein, the gp61 primase, and the gp32 single stranded DNA binding protein. A.) the gp32 single-stranded DNA binding protein binds to regions of displaced DNA near the replication fork. B.) the bacteriophage T7 gene 4 helicase domain is representative of the

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  • Response of the Bacteriophage T4 Replisome to Noncoding

    Sep 03, 2010 · Bacteriophage T4 helicase loader protein gp59 functions as gatekeeper in origin-dependent replication in vivo J. Biol. Chem., 280 ( 2005 ), pp. 21561 - 21569 Article Download PDF View Record in Scopus Google Scholar

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  • Pfam: Family: T4_Gp59_C (PF08994)

    T4 gene Gp59 loader of gp41 DNA helicase C-term Provide feedback Bacteriophage T4 gene-59 helicase assembly protein is required for recombination-dependent DNA replication, which is the predominant mode of DNA replication in the late stage of T4 infection.

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  • Interaction between the T4 Helicase-Loading Protein (gp59

    Jan 29, 2005 · Bacteriophage T4 Helicase Loader Protein gp59 Functions as Gatekeeper in Origin-dependent Replication in Vivo. Journal of Biological Chemistry 2005, 280 (22), 21561-21569. DOI: 10.1074/jbc.M502351200.

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  • Bacteriophage T4 Proteins Replicate Plasmids with a

    Whether there are any T4 origins that require 59 helicase loader has not been established in vivo. 59 protein, in vitro strongly stimulates synthesis from a preformed R loop within the T4 UvsY

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  • A Zinc Ribbon Protein in DNA Replication: Primer Synthesis

    Nov 21, 2001 · The gene product 61 primase protein from bacteriophage T4 was expressed as an intein fusion and purified to homogeneity. The primase binds one zinc ion, which is coordinated by four cysteine residues to form a zinc ribbon motif. Factors that influence the rate of priming were investigated, and a physiologically relevant priming rate of ∼1 primer per second per primosome was achieved.

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  • DNA Helicase–Polymerase Coupling in Bacteriophage DNA

    Jul 30, 2021 · The reconstitution of the T4 DNA replication elongation complex in vitro requires eight proteins: the gp43 DNA Pol, the gp45 clamp, the gp44/gp62 clamp loader, the gp41 Hel, the gp59 Hel loader, the gp61 primase, and the gp32 ssDNA-binding protein [48,49]. A holo-replicase complex (T4 Pol) comprises gp43 and gp45.

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  • Structural analysis of bacteriophage T4 DNA replication: a

    comparison to related proteins when the T4 structures are unavailable. Three of the ten full-length T4 replisomal proteins have been determined; the gp59 helicase loading protein, the RNase H, and the gp45 processivity clamp. The core of T4 gp32 and two proteins from the T4 related phage RB69, the gp43 polymerase and the gp45 clamp are also solved.

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  • Bacteriophage T4 Helicase Loader Protein gp59 Functions as

    Structural analysis of bacteriophage T4 DNA replication: a

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  • T4 Phage Replisome | SpringerLink

    May 02, 2009 · The bacteriophage T4 DNA replisome has been a useful model system for studying cellular DNA replication. Several decades of studies revealed that despite the variation in number and nature of individual proteins in the T4 replisome as compared to other model systems (Escherichia coli and yeast Saccharomyces cerevisiae), the fundamental components that constitute a functional …

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